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Activation of muscarinic cholinoceptor ameliorates tumor necrosis factor‐α‐induced barrier dysfunction in intestinal epithelial cells
Author(s) -
Khan Md Rafiqul Islam,
Uwada Junsuke,
Yazawa Takashi,
Islam Md Tariqul,
Krug Susanne M.,
Fromm Michael,
Karaki Shin-ichiro,
Suzuki Yuichi,
Kuwahara Atsukazu,
Yoshiki Hatsumi,
Sada Kiyonao,
Muramatsu Ikunobu,
Anisuzzaman Abu Syed Md,
Taniguchi Takanobu
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.10.029
Subject(s) - muscarinic acetylcholine receptor , barrier function , stimulation , tumor necrosis factor alpha , cytokine , chemistry , tight junction , microbiology and biotechnology , acetylcholine , medicine , endocrinology , immunology , biology , receptor
Impaired intestinal barrier function is one of the critical issues in inflammatory bowel diseases. The aim of this study is to investigate muscarinic cholinoceptor (mAChR)‐mediated signaling for the amelioration of cytokine‐induced barrier dysfunction in intestinal epithelium. Rat colon challenged with TNF‐α and interferon γ reduced transepithelial electrical resistance (TER). This barrier injury was attenuated by muscarinic stimulation. In HT‐29/B6 intestinal epithelial cells, muscarinic stimulation suppressed TNF‐α‐induced activation of NF‐κB signaling and barrier disruption. Finally, muscarinic stimulation promoted the shedding of TNFR1, which would be a mechanism for the attenuation of TNF‐α/NF‐κB signaling and barrier disruption via mAChR.