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Transcriptional analysis of the dachsous gene uncovers novel isoforms expressed during development in Drosophila
Author(s) -
Revilla-Yates Eva,
Varas Laura,
Sierra Javier,
Rodriguez Isabel
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.10.012
Subject(s) - gene knockdown , cadherin , gene isoform , microbiology and biotechnology , biology , gene , cell polarity , hippo signaling pathway , gene expression , regulation of gene expression , cytoplasm , genetics , signal transduction , cell
The Drosophila cadherin‐related protein Dachsous (Ds) plays a prominent role in planar cell polarity (PCP) and growth. The regulation of these two processes is based on the interaction between Ds and Fat proteins, generating an intracellular response required for tissue polarization and modulation of Hippo pathway activity. Here we have performed a comprehensive molecular study of the ds gene during larval development that has shown an unexpected complexity in its transcriptional regulation and revealed the expression of hitherto unsuspected transcripts. Also, knockdown of several isoforms provides new evidence on the importance of the cytoplasmic domain in the mechanism of action of Ds during development.