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Agonist mobility on supported lipid bilayers affects Fas mediated death response
Author(s) -
Sánchez M. Florencia,
Levi Valeria,
Weidemann Thomas,
Carrer Dolores C.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.10.009
Subject(s) - agonist , lipid bilayer , apoptosis , chemistry , microbiology and biotechnology , biophysics , receptor , transmembrane protein , membrane fluidity , fas ligand , membrane , programmed cell death , biochemistry , biology
Extrinsic apoptosis is initiated by recognition and clustering of the single‐pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas‐mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z‐scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer.