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Laminin therapy for the promotion of muscle regeneration
Author(s) -
Riederer Ingo,
Bonomo Adriana Cesar,
Mouly Vincent,
Savino Wilson
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.10.004
Subject(s) - laminin , regeneration (biology) , extracellular matrix , myocyte , microbiology and biotechnology , transplantation , dystrophin , skeletal muscle , biology , immunology , anatomy , medicine
Muscle regeneration is essentially due to activation of satellite cells, which can be isolated and amplified ex vivo, thus representing good candidates for cell therapy. Accumulating data show that the local microenvironment plays a major role during muscle regeneration. In the satellite cell niche, a major extracellular matrix protein is laminin. Human myoblasts transplanted into immunodeficient mice are preferentially located in laminin‐enriched areas. Additionally, laminin‐111 enhances myoblast proliferation in vitro and increases expression of the α7β1 integrin‐type laminin receptor. Intramuscular injection of laminin‐111 ameliorates muscular pathology in mdx mice, protecting muscle fibers from damage. Moreover, transplantation of human myoblasts with laminin‐111 into Rag/mdx immunodeficient recipients improved efficacy of myoblast transplantation, increasing the number of human dystrophin‐positive myofibres. Taken together, these data strongly indicate that exogenous laminin can ameliorate the regeneration process in different models of muscular dystrophies and can be instrumental for improving cell therapy aiming at repairing the degeneration/regeneration process in skeletal muscle.