Premium
MicroRNA‐145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb
Author(s) -
Fukuda Toru,
Ochi Hiroki,
Sunamura Satoko,
Haiden Akina,
Bando Waka,
Inose Hiroyuki,
Okawa Atsushi,
Asou Yoshinori,
Takeda Shu
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.09.024
Subject(s) - runx2 , core binding factor , transcription factor , microbiology and biotechnology , microrna , osteoblast , endogeny , regulator , chemistry , cellular differentiation , biology , gene , biochemistry , in vitro
Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt‐related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero‐dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR‐145 as a crucial regulator of Cbfb expression. The expression of miR‐145 increased during osteoblastogenesis, indicating that miR‐145 works as an inhibitor of osteoblastogenesis. Stable expression of miR‐145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR‐34c. Furthermore, miR‐145 decreased bone regeneration in vivo. Our results indicate that miR‐145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network.