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Down‐regulated expression of miR‐134 contributes to paclitaxel resistance in human ovarian cancer cells
Author(s) -
Shuang Ting,
Wang Min,
Shi Cong,
Zhou Yingying,
Wang Dandan
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.08.047
Subject(s) - paclitaxel , apoptosis , microrna , cancer research , ovarian cancer , psychological repression , phosphorylation , chemistry , cancer , biology , medicine , microbiology and biotechnology , gene expression , gene , biochemistry
MiR‐134 has been reported to have a role in the development and progression of various cancers. In this study, we found that miR‐134 expression was significantly decreased in chemo‐resistant serous epithelial ovarian cancer (EOC) patients. Over‐expression of miR‐134 enhanced the sensitivity of SKOV3‐TR30 cells to paclitaxel, and increased paclitaxel‐induced apoptosis. Further, Pak2 was identified as a direct target of miR‐134, and Pak2‐specific siRNA increased cell inhibition rate and promoted paclitaxal‐induced apoptosis. By regulating Pak2 expression, miR‐134 could mediate Bad phosphorylation at Ser112 and Ser136, which affected cell survival and apoptosis. In conclusion, our findings indicate that repression of miR‐134 and consequent up‐regulation of Pak2 might contribute to paclitaxel resistance.