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Misfolded opsin mutants display elevated β ‐sheet structure
Author(s) -
Miller Lisa M.,
Gragg Megan,
Kim Tae Gyun,
Park Paul S.-H.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.08.042
Subject(s) - opsin , endoplasmic reticulum , rhodopsin , mutant , retinitis pigmentosa , retinal degeneration , chemistry , biophysics , microbiology and biotechnology , biology , mutation , retinal , biochemistry , gene
Mutations in rhodopsin can cause misfolding and aggregation of the receptor, which leads to retinitis pigmentosa, a progressive retinal degenerative disease. The structure adopted by misfolded opsin mutants and the associated cell toxicity is poorly understood. Förster resonance energy transfer (FRET) and Fourier transform infrared (FTIR) microspectroscopy were utilized to probe within cells the structures formed by G188R and P23H opsins, which are misfolding mutants that cause autosomal dominant retinitis pigmentosa. Both mutants formed aggregates in the endoplasmic reticulum and exhibited altered secondary structure with elevated β ‐sheet and reduced α ‐helical content. The newly formed β ‐sheet structure may facilitate the aggregation of misfolded opsin mutants. The effects observed for the mutants were unrelated to retention of opsin molecules in the endoplasmic reticulum itself.

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