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Borate–fructose complex: A novel mediator for laccase and its new function for fructose determination
Author(s) -
Cheng Chih-Yu,
Liao Chia-I.,
Lin Shuen-Fuh
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.08.032
Subject(s) - laccase , fructose , chemistry , boron , biochemistry , fermentation , glucose oxidase , enzyme , organic chemistry
Laccase and borate–fructose complex were investigated by coincidence in a solid‐state fermentation of Edenia sp. TS‐76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34‐fold purification and 32% yield. Fructose had no significant effect on laccase activity, whereas borate reduced laccase activity by 60–90%; conversely, the borate–fructose complex increased laccase activity by nearly fourfold at pH 7.5. The complex caused a shift in the optimal pH for laccase from 5.0 to 7.5 and served as a highly efficient mediator. Borate complexed with fructose provides an alternative, time‐saving, and specific method for serum fructose determination.