Wnts grasp the WIF domain of Wnt Inhibitory Factor 1 at two distinct binding sites | Zendy

Kerekes Krisztina | Zendy; Bányai László | Zendy; Patthy László | Zendy

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Wnts grasp the WIF domain of Wnt Inhibitory Factor 1 at two distinct binding sites

Author(s) -

Kerekes Krisztina,

Bányai László,

Patthy László

Publication year - 2015

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2015.08.031

Subject(s) - grasp , inhibitory postsynaptic potential , wnt signaling pathway , factor (programming language) , domain (mathematical analysis) , computational biology , chemistry , neuroscience , computer science , biology , biochemistry , mathematics , signal transduction , mathematical analysis , programming language

Wnts have a structure resembling a hand with “thumb” and “index” fingers that grasp the cysteine rich domains of Frizzled receptors at two distinct binding sites. In the present work we show that the WIF domain of Wnt Inhibitory Factor 1 is also bound by Wnts at two sites. Using C‐terminal domains of Wnt5a and Wnt7a and arginine‐scanning mutagenesis of the WIF domain we demonstrate that, whereas the N‐terminal, lipid‐modified “thumb” of Wnts interacts with the alkyl‐binding site of the WIF domain, the C‐terminal domain of Wnts (Wnt‐CTD) binds to a surface on the opposite side of the WIF domain.

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