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Bacterial cell motility of Burkholderia gut symbiont is required to colonize the insect gut
Author(s) -
Lee Jun Beom,
Byeon Jin Hee,
Jang Ho Am,
Kim Jiyeun Kate,
Yoo Jin Wook,
Kikuchi Yoshitomo,
Lee Bok Luel
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.08.022
Subject(s) - mutant , biology , burkholderia , motility , microbiology and biotechnology , symbiosis , peptidoglycan , burkholderia pseudomallei , insect , phenotype , bacteria , gene , biochemistry , genetics , botany
We generated a Burkholderia mutant, which is deficient of an N ‐acetylmuramyl‐ l ‐alanine amidase, AmiC, involved in peptidoglycan degradation. When non‐motile Δ amiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, Δ amiC mutant cells were unable to establish symbiotic association. But, Δ amiC mutant complemented with amiC gene restored in vivo symbiotic association. Δ amiC mutant cultured in minimal medium restored their motility with single‐celled morphology. When Δ amiC mutant cells harboring single‐celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont.