Premium
Inhibition of Yap2 activity by MAPKAP kinase Rck1 affects yeast tolerance to cadmium
Author(s) -
Mazzola Daiane,
Pimentel Catarina,
Caetano Soraia,
Amaral Catarina,
Menezes Regina,
Santos Claudia N.,
Eleutherio Elis,
Rodrigues-Pousada Claudina
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.049
Subject(s) - cadmium , yeast , yap1 , microbiology and biotechnology , mapk/erk pathway , oxidative stress , protein kinase a , chemistry , transcription factor , kinase , gene , biology , biochemistry , organic chemistry
Yap2 is a cadmium responsive transcription factor that interacts with MAPK‐activated protein (MAPKAP) kinase Rck1. We show that Rck1 deletion confers protection against cadmium toxicity and that the mechanism underlying this observation relies on Yap2. Rck1 removal from the yeast genome potentiates Yap2 activity by increasing protein half‐life and delaying its nuclear export. As a consequence, several Yap2 antioxidant targets are over‐activated by a mechanism that also requires Yap1. Several genes of the cell wall integrity (CWI) pathway are upregulated under cadmium stress in a Yap2 dependent way. We showed that deletion of CWI genes renders yeast cells more sensitive to cadmium. These findings led us to suggest that in response to cadmium stress Yap2 may serve a dual purpose: oxidative stress attenuation and cell wall maintenance.