Premium
CLIP‐170 tethers kinetochores to microtubule plus ends against poleward force by dynein for stable kinetochore–microtubule attachment
Author(s) -
Amin Mohammed Abdullahel,
Kobayashi Kinue,
Tanaka Kozo
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.036
Subject(s) - kinetochore , microtubule , dynactin , microbiology and biotechnology , dynein , biology , chemistry , genetics , chromosome , gene
The cytoplasmic linker protein (CLIP)‐170 localizes to kinetochores and is suggested to function in stable attachment of kinetochores to microtubule ends. Here we show that defects in kinetochore–microtubule attachment and chromosome alignment in CLIP‐170‐depleted cells were rescued by co‐depletion of p150glued, a dynactin subunit required for kinetochore localization of CLIP‐170. CLIP‐170 recruited p150glued to microtubule ends. Kinetochore localization at microtubule ends was perturbed by CLIP‐170 depletion, which was rescued by co‐depleting p150glued. Our results imply that CLIP‐170 tethers kinetochores to microtubule ends against the dynein‐mediated poleward force to slide kinetochores along microtubules, facilitating the stable kinetochore attachment to microtubules.