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Hypoxia inducible factor 1α down regulates cell surface expression of α1,2‐fucosylated glycans in human pancreatic adenocarcinoma cells
Author(s) -
Belo Ana I.,
van Vliet Sandra J.,
Maus Andreas,
Laan Lisa C.,
Nauta Tessa D.,
Koolwijk Pieter,
Tefsen Boris,
van Die Irma
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.035
Subject(s) - fucosylation , fucosyltransferase , cell culture , chemistry , hypoxia inducible factors , cancer research , cell , pancreatic cancer , microbiology and biotechnology , glycan , biology , gene , biochemistry , cancer , glycoprotein , genetics
The α1,2‐fucosyltransferase activity in pancreatic tumors is much lower compared to normal pancreatic tissue. Here we show that hypoxia inducible factor (HIF) 1α is constitutively expressed in the pancreatic cancer cell lines Pa‐Tu‐8988S and Pa‐Tu‐8988T and suppresses the expression of the α1,2‐fucosyltransferase genes FUT1 and FUT2 . Down regulation of HIF‐1α expression resulted in elevated FUT1 and FUT2 transcript levels and an increased expression of α1,2‐fucosylated glycan structures on the surface of these cells. In conclusion, our data are the first to identify HIF‐1α as a suppressor of FUT1/2 expression, thereby regulating α1,2‐fucosylation of cell‐surface glycans.