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Glucuronoyl esterases are active on the polymeric substrate methyl esterified glucuronoxylan
Author(s) -
Biely Peter,
Malovíková Anna,
Uhliariková Iveta,
Li Xin-Liang,
Wong Dominic W.S.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.019
Subject(s) - trichoderma reesei , chemistry , substrate (aquarium) , schizophyllum commune , methanol , organic chemistry , hydrolysis , lignin , cell wall , biochemistry , cellulase , biology , ecology
Alkali extracted beechwood glucuronoxylan methyl ester prepared by esterification of 4‐ O ‐methyl‐ d ‐glucuronic acid side residues by methanol was found to serve as substrate of microbial glucuronoyl esterases from Ruminococcus flavefaciens , Schizophyllum commune and Trichoderma reesei . The enzymatic deesterification was monitored by 1 H NMR spectroscopy and evaluated on the basis of the decrease of the signal of the ester methyl group and increase of the signal of methanol. The results show for the first time the action of enzymes on polymeric substrate, which imitates more closely the natural substrate in plant cell walls than the low molecular mass artificial substrates used up to present.