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PKA, PHO and stress response pathways regulate the expression of UDP‐glucose pyrophosphorylase through Msn2/4 in budding yeast
Author(s) -
Yi Dae-Gwan,
Huh Won-Ki
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.015
Subject(s) - saccharomyces cerevisiae , transcription factor , yeast , microbiology and biotechnology , protein kinase a , chemistry , biochemistry , enzyme , signal transduction , transcription (linguistics) , biology , gene , linguistics , philosophy
Ugp1, a UDP‐glucose pyrophosphorylase, is essential for various cellular activities in Saccharomyces cerevisiae because its product, UDP‐glucose, is a sole glucosyl donor in several metabolic pathways. Here, we report that Msn2/4 play a crucial role in the regulation of UGP1 expression. Msn2/4 bound to three stress response elements in the UGP1 promoter in a protein kinase A (PKA)‐dependent manner. Several stresses induced UGP1 transcription, suggesting that the regulation of UGP1 mediated by Msn2/4 is involved in general stress response. Furthermore, the phosphate response (PHO) pathway also controlled Msn2/4‐dependent regulation of UGP1 , providing a novel link between the PKA and PHO pathways. Our data suggest that signals of the PKA, PHO and stress response pathways regulate UGP1 expression via Msn2/4 in S. cerevisiae .