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Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
Author(s) -
Hernandez-Gomez Mercedes C.,
Rydahl Maja G.,
Rogowski Artur,
Morland Carl,
Cartmell Alan,
Crouch Lucy,
Labourel Aurore,
Fontes Carlos M.G.A.,
Willats William G.T.,
Gilbert Harry J.,
Knox J. Paul
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.07.009
Subject(s) - xyloglucan , cellulose , carbohydrate binding module , polysaccharide , chemistry , xylose , carbohydrate , glucan , cell wall , biochemistry , stereochemistry , crystallography , cellulase , fermentation
Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed.