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Cellular stress induces cap‐independent alpha‐enolase/MBP‐1 translation
Author(s) -
Maranto Cristina,
Perconti Giovanni,
Contino Flavia,
Rubino Patrizia,
Feo Salvatore,
Giallongo Agata
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.06.030
Subject(s) - enolase , microbiology and biotechnology , signal transduction , biology , protein kinase b , translation (biology) , alpha (finance) , messenger rna , biochemistry , immunology , gene , medicine , construct validity , immunohistochemistry , nursing , patient satisfaction
Myc promoter‐binding protein‐1 (MBP‐1) is a shorter protein variant of the glycolytic enzyme alpha‐enolase. Although several lines of evidence indicate that MBP‐1 acts as a tumor suppressor, the cellular mechanisms and signaling pathways underlying MBP‐1 expression still remain largely elusive. To dissect these pathways, we used the SkBr3 breast cancer cell line and non‐tumorigenic HEK293T cells ectopically overexpressing alpha‐enolase/MBP‐1. Here, we demonstrate that induced cell stresses promote MBP‐1 expression through the AKT/PERK/eIF2α signaling axis. Our results contribute to shedding light on the molecular mechanisms underlying MBP‐1 expression in non‐tumorigenic and cancer cells.