Premium
Suppression of ornithine decarboxylase promotes osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells
Author(s) -
Tsai Yo-Hsian,
Lin Kuan-Lian,
Huang Yuan-Pin,
Hsu Yi-Chiang,
Chen Chung-Hwan,
Chen Yuhsin,
Sie Min-Hua,
Wang Gwo-Jaw,
Lee Mon-Juan
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.06.023
Subject(s) - ornithine decarboxylase , adipogenesis , mesenchymal stem cell , polyamine , alkaline phosphatase , microbiology and biotechnology , chemistry , bone marrow , stem cell , cellular differentiation , biology , biochemistry , enzyme , immunology , gene
Ornithine decarboxylase (ODC) is the rate‐limiting enzyme for polyamine biosynthesis. Suppression of ODC by its irreversible inhibitor, α‐difluoromethylornithine (DFMO), or by RNA interference through siRNA, enhanced osteogenic gene expression and alkaline phosphatase activity, and accelerated matrix mineralization of human bone marrow‐derived mesenchymal stem cells (hBMSCs). Besides, adipogenic gene expression and lipid accumulation was attenuated, indicating that the enhanced osteogenesis was accompanied by down‐regulation of adipogenesis when ODC was suppressed. A decrease in the intracellular polyamine content of hBMSCs during osteogenic induction was observed, suggesting that the level of endogenous polyamines is regulated during differentiation of hBMSCs. This study elucidates the role of polyamine metabolism in the lineage commitment of stem cells and provides a potential new indication for DFMO as bone‐stimulating drug.