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Nuclear versus cytosolic activity of the yeast Hog1 MAP kinase in response to osmotic and tunicamycin‐induced ER stress
Author(s) -
García-Marqués Sara,
Randez-Gil Francisca,
Prieto Jose A.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.06.021
Subject(s) - osmotic shock , tunicamycin , cytosol , osmolyte , endoplasmic reticulum , microbiology and biotechnology , biology , yeast , mapk/erk pathway , biochemistry , phosphorylation , unfolded protein response , enzyme , gene
We examined the physiological significance of the nuclear versus cytosolic localization of the MAPK Hog1p in the ability of yeast cells to cope with osmotic and ER (endoplasmic reticulum) stress. Our results indicate that nuclear import of Hog1p is not critical for osmoadaptation. Plasma membrane‐anchored Hog1p is still able to induce increased expression of GPD1 and glycerol accumulation. This is a key osmoregulatory event, although a small production of the osmolyte coupled with the nuclear import of Hog1p is sufficient to provide osmoresistance. On the contrary, the nuclear activity of Hog1p is dispensable for ER stress adaptation.