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Faithful expression of imprinted genes in donor cells of SCNT cloned pigs
Author(s) -
Wang Dongxu,
Yuan Lin,
Sui Tingting,
Song Yuning,
Lv Qingyan,
Wang Anfeng,
Li Zhanjun,
Lai Liangxue
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.06.016
Subject(s) - somatic cell nuclear transfer , genomic imprinting , cloning (programming) , biology , microbiology and biotechnology , gene , dna methylation , imprinting (psychology) , somatic cell , methylation , embryo , gene expression , genetics , andrology , blastocyst , embryogenesis , medicine , computer science , programming language
To understand if the genomic imprinting status of the donor cells is altered during the process of SCNT (somatic cell nuclear transfer), cloned pigs were produced by SCNT using PEF (porcine embryonic fibroblast) and P‐PEF (parthenogenetic‐PEF) cells as donors. Then, the gene expression and methylation patterns of H19 , IGF2 , NNAT and MEST were compared between PEF vs . C‐PEF (cloned‐PEF), P‐PEF vs . CP‐PEF (cloned‐P‐PEF), respectively. Taken together, the results revealed that there was no significant difference in the expression of imprinted genes and conserved genomic imprints between the donor and cloned cells.