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ZNF10 inhibits HIV‐1 LTR activity through interaction with NF‐κB and Sp1 binding motifs
Author(s) -
Nishitsuji Hironori,
Sawada Leila,
Sugiyama Ryuichi,
Takaku Hiroshi
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.06.013
Subject(s) - repressor , psychological repression , zinc finger , biology , gene , long terminal repeat , krüppel , genetics , regulation of gene expression , genome , microbiology and biotechnology , gene expression , transcription factor
Kruppel‐associated box‐containing zinc finger (KRAB‐ZNF) genes constitute the single largest gene family of transcriptional repressors in the genomes of higher organisms. In this study, we isolated 52 cDNA clones of KRAB‐ZFPs from U1 cell lines and screened them to identify which were capable of regulating HIV‐1 gene expression. We identified 5 KRAB‐ZFPs that suppressed ⩾50% of HIV‐1 LTR. Of the 5 identified KRAB‐ZFPs, the expression of ZNF10 significantly enhanced the transcriptional repression activity of the LTR compared with other ZNFs. In addition, the depletion of endogenous ZNF10 led to the activation of HIV‐1 LTR. The repressor activity of ZNF10 was required for TRIM28, SETDB1 and HP1‐gamma binding. These results indicate that ZNF10 could be involved in a potent intrinsic antiretroviral defense.