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miR‐362‐5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3‐kinase‐C2β
Author(s) -
Wu Kai,
Yang Liucheng,
Chen Jianfeng,
Zhao Haijun,
Wang Jianjun,
Xu Shuai,
Huang Zonghai
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.05.056
Subject(s) - phosphatidylinositol , neuroblastoma , kinase , microbiology and biotechnology , chemistry , cancer research , biology , cell culture , genetics
miR‐362‐5p is down‐regulated in high‐risk neuroblastoma and can function as a tumor suppressor. However, its role remains poorly understood. We show that miR‐362‐5p is down‐regulated in metastatic neuroblastoma compared with primary neuroblastoma. Overexpression of miR‐362‐5p inhibits cell proliferation, migration and invasion of neuroblastoma cells in vitro and suppresses tumor growth of neuroblastoma in vivo. Phosphatidylinositol 3‐kinase (PI3K)‐C2β is a target of miR‐362‐5p. Knockdown of PI3K‐C2β by siRNA had a similar effect to overexpression of miR‐362‐5p on SH‐SY5Y cells. Overexpression of PI3K‐C2β partially reversed tumor‐suppressive effects of miR‐362‐5p. We suggest that miR‐362‐5p suppresses neuroblastoma cell growth and motility, partially by targeting PI3K‐C2β.