Premium
Cross‐linking analysis reveals the putative dimer structure of the cyanobacterial BLUF photoreceptor PixD
Author(s) -
Ren Shukun,
Sugimoto Yuki,
Kobayashi Taichi,
Masuda Shinji
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.05.019
Subject(s) - dimer , photosensitivity , phototaxis , chemistry , halobacteriaceae , trimer , crystal structure , crystallography , stereochemistry , biophysics , photochemistry , halobacterium salinarum , biology , biochemistry , physics , optics , botany , bacteriorhodopsin , organic chemistry , membrane
PixD is a blue light using flavin (BLUF)‐type blue‐light photoreceptor controlling phototaxis in the cyanobacterium Synechocystis sp. PCC6803. The crystal structure of PixD shows a decamer, although in solution an equilibrium is maintained between the dimer and decamer. Because the ratio of these two conformers is altered by illumination, the equilibrium state determines photosensitivity. However, no structural information is available for the PixD dimer. Here, we report a predicted structure for the dimer based on docking simulation, mutagenesis, and mass spectrometry‐based cross‐linking analyses. The results indicate the importance of the PixD C‐terminus for dimer preference and photosensitivity.