Premium
Membrane protein production in Escherichia coli cell‐free lysates
Author(s) -
Henrich Erik,
Hein Christopher,
Dötsch Volker,
Bernhard Frank
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.04.045
Subject(s) - cell free protein synthesis , escherichia coli , synthetic biology , membrane protein , protein biosynthesis , biology , membrane , microbiology and biotechnology , biochemistry , cell , chemistry , computational biology , gene
Cell‐free protein production has become a core technology in the rapidly spreading field of synthetic biology. In particular the synthesis of membrane proteins, highly problematic proteins in conventional cellular production systems, is an ideal application for cell‐free expression. A large variety of artificial as well as natural environments for the optimal co‐translational folding and stabilization of membrane proteins can rationally be designed. The high success rate of cell‐free membrane protein production allows to focus on individually selected targets and to modulate their functional and structural properties with appropriate supplements. The efficiency and robustness of lysates from Escherichia coli strains allow a wide diversity of applications and we summarize current strategies for the successful production of high quality membrane protein samples.