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Opposite expression of CYP51A1 and its natural antisense transcript AluCYP51A1 in adenovirus type 37 infected retinal pigmented epithelial cells
Author(s) -
Pickl Julia Maria Anna,
Kamel Wael,
Ciftci Sibel,
Punga Tanel,
Akusjärvi Göran
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.04.018
Subject(s) - retinal pigment epithelium , biology , gene expression , retinal , antisense rna , microbiology and biotechnology , messenger rna , downregulation and upregulation , gene , regulation of gene expression , rna , promoter , genetics , biochemistry
Cytochrome P450 family member CYP51A1 is a key enzyme in cholesterol biosynthesis whose deregulation is implicated in numerous diseases, including retinal degeneration. Here we describe that HAdV‐37 infection leads to downregulation of CYP51A1 expression and overexpression of its antisense non‐coding Alu element ( AluCYP51A1 ) in retinal pigment epithelium (RPE) cells. This change in gene expression is associated with a reversed accumulation of a positive histone mark at the CYP51A1 and AluCYP51A1 promoters. Further, transient AluCYP51A1 RNA overexpression correlates with reduced CYP51A1 mRNA accumulation. Collectively, our data suggest that AluCYP51A1 might control CYP51A1 gene expression in HAdV‐37‐infected RPE cells.