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Standalone cohesin as a molecular shuttle in cellulosome assembly
Author(s) -
Voronov-Goldman Milana,
Yaniv Oren,
Gul Ozgur,
Yoffe Hagar,
Salama-Alber Orly,
Slutzki Michal,
Levy-Assaraf Maly,
Jindou Sadanari,
Shimon Linda J.W.,
Borovok Ilya,
Bayer Edward A.,
Lamed Raphael,
Frolow Felix
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.04.013
Subject(s) - cellulosome , cohesin , microbiology and biotechnology , biology , chemistry , biochemistry , clostridium thermocellum , dna , cellulase , enzyme , chromatin
The cellulolytic bacterium Ruminococcus flavefaciens of the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin‐bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin‐borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.