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Protein kinase A signaling and calcium ions are major players in PAF mediated toxicity against Aspergillus niger
Author(s) -
Binder Ulrike,
Benčina Mojca,
Fizil Ádám,
Batta Gyula,
Chhillar Anil K.,
Marx Florentine
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.03.037
Subject(s) - aspergillus niger , aequorin , mode of action , biochemistry , calcium , protein kinase a , biology , calcium signaling , kinase , signal transduction , chemistry , microbiology and biotechnology , intracellular , organic chemistry
The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin‐expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca 2+ dynamics in response to PAF. This Ca 2+ signature depended on an intact positively charged lysine‐rich PAF motif. By combining Ca 2+ measurements in A. niger mutants with deregulated cAMP/protein kinase A (PKA) signaling, we proved the interconnection of Ca 2+ perturbation and cAMP/PKA signaling in the mechanistic function of PAF. A deep understanding of the mode of action of PAF is an invaluable prerequisite for its future application as new antifungal drug.