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Interaction between Bacillus subtilis YsxC and ribosomes (or rRNAs)
Author(s) -
Wicker-Planquart Catherine,
Jault Jean-Michel
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.03.001
Subject(s) - bacillus subtilis , ribosome , chemistry , microbiology and biotechnology , biology , biochemistry , genetics , bacteria , rna , gene
The stoichiometry of YsxC ribosome subunit complex was evaluated. We showed that YsxC binding to the 50S ribosomal subunit is not affected by GTP, but in the presence of GDP the stoichiometry of YsxC‐ribosome is decreased. YsxC GTPase activity was stimulated upon 50S ribosomal subunit binding. In addition, it is shown for the first time that YsxC binds both 16S and 23S ribosomal RNAs.