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Identification of minimum Rpn4‐responsive elements in genes related to proteasome functions
Author(s) -
Shirozu Ryohei,
Yashiroda Hideki,
Murata Shigeo
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.02.025
Subject(s) - identification (biology) , proteasome , gene , chemistry , computational biology , biology , biochemistry , ecology
The proteasome is an essential, 66‐subunit protease that mediates ubiquitin‐dependent proteolysis. The transcription factor Rpn4 regulates concerted expression of proteasome subunits to increase the proteasome by recognizing nonamer proteasome‐associated control element (PACE) elements on the promoter regions. However, the genes for proteasome assembly chaperones and some of the subunits have no PACEs. Here we identified a minimal hexamer “PACE‐core” sequence that responds to Rpn4. PACE‐cores are found in many genes related to proteasome function including the assembly chaperones, but cannot substitute for PACE of the subunits. Our results add a new layer of complexity in transcriptional regulation of genes involved in protein degradation.