Experimental evidence suggesting that H 2 O 2  is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen | Zendy

Khorobrykh Sergey A. | Zendy; Karonen Maarit | Zendy; Tyystjärvi Esa | Zendy

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Experimental evidence suggesting that H 2 O 2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen

Author(s) -

Khorobrykh Sergey A.,

Karonen Maarit,

Tyystjärvi Esa

Publication year - 2015

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2015.02.011

Subject(s) - plastoquinone , thylakoid , singlet oxygen , chemistry , membrane , electron transport chain , photochemistry , chromatography , methanol , electron transfer , oxygen , biophysics , chloroplast , biochemistry , biology , organic chemistry , gene

Plastoquinol (PQH 2 ‐9) and plastoquinone (PQ‐9) mediate photosynthetic electron transfer. We isolated PQH 2 ‐9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH 2 ‐9 to singlet oxygen ( 1 O 2 ) and analyzed the products. The main reaction of 1 O 2 with PQH 2 ‐9 in methanol was found to result in formation of PQ‐9 and H 2 O 2 , and the amount of H 2 O 2 produced was essentially the same as the amount of oxidized PQH 2 ‐9. Formation of H 2 O 2 in the reaction between 1 O 2 and PQH 2 ‐9 may be an important source of H 2 O 2 within the lipophilic thylakoid membrane.

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