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Localization of Atg3 to autophagy‐related membranes and its enhancement by the Atg8‐family interacting motif to promote expansion of the membranes
Author(s) -
Sakoh-Nakatogawa Machiko,
Kirisako Hiromi,
Nakatogawa Hitoshi,
Ohsumi Yoshinori
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.02.003
Subject(s) - atg8 , membrane , autophagy , microbiology and biotechnology , saccharomyces cerevisiae , autophagosome , ubiquitin , chemistry , biochemistry , biology , gene , apoptosis
The E2 enzyme Atg3 conjugates the ubiquitin‐like protein Atg8 to phosphatidylethanolamine (PE) to drive autophagosome formation in Saccharomyces cerevisiae . In this study, we show that Atg3 localizes to the pre‐autophagosomal structure (PAS) and the isolation membrane (IM), providing crucial evidence that Atg8‐PE conjugates are produced on these structures. We also find that mutations in the Atg8‐family interacting motif (AIM) of Atg3 significantly impairs the PAS/IM localization of Atg3, resulting in inefficient IM expansion. It is suggested that the AIM‐mediated PAS/IM localization of Atg3 facilitates membrane expansion in these structures probably by ensuring active production of Atg8‐PE on the membranes.