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Pirin regulates epithelial to mesenchymal transition independently of Bcl3‐Slug signaling
Author(s) -
Komai Kuniya,
Niwa Yuki,
Sasazawa Yukiko,
Simizu Siro
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.01.040
Subject(s) - slug , epithelial–mesenchymal transition , cadherin , microbiology and biotechnology , hela , mutant , signal transduction , metastasis , cancer research , chemistry , biology , cancer , cell culture , biochemistry , gene , genetics , cell
Epithelial to mesenchymal transition (EMT) is an important mechanism for the initial step of metastasis. Proteomic analysis indicates that Pirin is involved in metastasis. However, there are no reports demonstrating its direct contribution. Here we investigated the involvement of Pirin in EMT. In HeLa cells, Pirin suppressed E‐cadherin expression and regulated the expression of other EMT markers. Furthermore, cells expressing Pirin exhibited a spindle‐like morphology, which is reminiscent of EMT. A Pirin mutant defective for Bcl3 binding decreased E‐cadherin expression similar to wild‐type, suggesting that Pirin regulates E‐cadherin independently of Bcl3‐Slug signaling. These data provide direct evidence that Pirin contributes to cancer metastasis.