Premium
Redox‐dependent translocation of the heat shock transcription factor AtHSFA8 from the cytosol to the nucleus in Arabidopsis thaliana
Author(s) -
Giesguth Miriam,
Sahm Arne,
Simon Swen,
Dietz Karl-Josef
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.01.039
Subject(s) - transcription factor , heat shock factor , cytosol , microbiology and biotechnology , arabidopsis , chromosomal translocation , nucleus , in silico , heat shock protein , biology , arabidopsis thaliana , chemistry , transcription (linguistics) , biochemistry , mutant , gene , hsp70 , linguistics , philosophy , enzyme
The hypothesis is tested that some heat stress transcription factors (HSFs) are activated after formation of inter‐ or intramolecular disulfide bonds. Based on in silico analyses we identified conserved cysteinyl residues in AtHSFA8 that might function as redox sensors in plants. AtHSFA8 represents a redox‐sensitive transcription factor since upon treatment of protoplasts with H 2 O 2 YFP‐labeled HSFA8 was translocated to the nucleus in a time‐dependent manner. Site‐directed mutagenesis of the conserved residues Cys24 and Cys269 blocked translocation of HSFA8 to the nucleus. The findings concur with a model where HSFA8 functions as redox sensing transcription factor within the stress‐responsive transcriptional network.