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Structural insights into Cn ‐AMP1, a short disulfide‐free multifunctional peptide from green coconut water
Author(s) -
Santana Mábio J.,
de Oliveira Aline L.,
Queiroz Júnior Luiz H.K.,
Mandal Santi M.,
Matos Carolina O.,
de O. Dias Renata,
Franco Octavio L.,
Lião Luciano M.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.01.029
Subject(s) - peptide , disulfide bond , cationic polymerization , random coil , chemistry , antibacterial peptide , flexibility (engineering) , combinatorial chemistry , biochemistry , protein secondary structure , biology , organic chemistry , bacteria , antibacterial activity , statistics , mathematics , genetics
Multifunctional and promiscuous antimicrobial peptides (AMPs) can be used as an efficient strategy to control pathogens. However, little is known about the structural properties of plant promiscuous AMPs without disulfide bonds. CD and NMR were used to elucidate the structure of the promiscuous peptide Cn ‐AMP1, a disulfide‐free peptide isolated from green coconut water. Data here reported shows that peptide structure is transitory and could be different according to the micro‐environment. In this regard, Cn ‐AMP1 showed a random coil in a water environment and an α‐helical structure in the presence of SDS‐d 25 micelles. Moreover, deuterium exchange experiments showed that Gly4, Arg5 and Met9 residues are less accessible to solvent, suggesting that flexibility and cationic charges seem to be essential for Cn ‐AMP1 multiple activities.