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The mitochondrial dicarboxylate and 2‐oxoglutarate carriers do not transport glutathione
Author(s) -
Booty Lee M.,
King Martin S.,
Thangaratnarajah Chancievan,
Majd Homa,
James Andrew M.,
Kunji Edmund R.S.,
Murphy Michael P.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.01.027
Subject(s) - glutathione , mitochondrial carrier , cytosol , mitochondrion , biochemistry , lactococcus lactis , inner mitochondrial membrane , chemistry , transporter , biology , enzyme , bacteria , bacterial outer membrane , lactic acid , gene , genetics , escherichia coli
Glutathione carries out vital protective roles within mitochondria, but is synthesised in the cytosol. Previous studies have suggested that the mitochondrial dicarboxylate and 2‐oxoglutarate carriers were responsible for glutathione uptake. We set out to characterise the putative glutathione transport by using fused membrane vesicles of Lactococcus lactis overexpressing the dicarboxylate and 2‐oxoglutarate carriers. Although transport of the canonical substrates could be measured readily, an excess of glutathione did not compete for substrate uptake nor could transport of glutathione be measured directly. Thus these mitochondrial carriers do not transport glutathione and the identity of the mitochondrial glutathione transporter remains unknown.