Premium
Dynamic subcellular localization of aquaporin‐7 in white adipocytes
Author(s) -
Miyauchi Takayuki,
Yamamoto Hiroyuki,
Abe Yoichiro,
Yoshida Go J.,
Rojek Aleksandra,
Sohara Eisei,
Uchida Shinichi,
Nielsen Søren,
Yasui Masato
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2015.01.025
Subject(s) - subcellular localization , aquaporin , white (mutation) , aquaporin 1 , chemistry , microbiology and biotechnology , biology , biochemistry , water channel , geology , gene , oceanography , inlet
Aquaporin‐7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7‐null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re‐localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI‐58). This relocation was confirmed by functional studies in 3T3‐L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes.