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Hyaluronan inhibits BMP‐induced osteoblast differentiation
Author(s) -
Kaneko Keiko,
Higuchi Chikahisa,
Kunugiza Yasuo,
Yoshida Kiyoshi,
Sakai Takashi,
Yoshikawa Hideki,
Nakata Ken
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.12.031
Subject(s) - osteoblast , c2c12 , chemistry , bone morphogenetic protein 2 , microbiology and biotechnology , bone morphogenetic protein , cd44 , phosphorylation , cellular differentiation , receptor , endocrinology , biology , biochemistry , cell , in vitro , myogenesis , gene
Hyaluronan (HA), one of the major structural extracellular components in cartilage, regulates cellular responses via receptors such as CD44. However, the direct effects of HA on osteoblastic differentiation has not been studied in detail. Here, we investigated the effects of HA (molecular weight: 900–1200kDa) on osteoblastic differentiation that was induced by bone morphogenetic protein (BMP) in C2C12 cells (mouse myoblastic cells) and ST2 cells (mouse bone marrow cells). BMP‐induced osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation were downregulated by HA. Use of the CD44‐blocking antibody restored HA‐induced inhibition of osteoblastic differentiation and Smad1/Smad5/Smad8 phosphorylation. Our results indicate that HA inhibits BMP‐induced osteoblastic differentiation through the CD44 receptor.