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Effects of detyrosinated tubulin on Na + ,K + ‐ATPase activity and erythrocyte function in hypertensive subjects
Author(s) -
Amaiden Marina R.,
Santander Verónica S.,
Monesterolo Noelia E.,
Nigra Ayelen D.,
Rivelli Juan F.,
Campetelli Alexis N.,
Pie Juan,
Casale Cesar H.
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.12.022
Subject(s) - tubulin , acetylation , atpase , tyrosine , enzyme , chemistry , medicine , endocrinology , biochemistry , biology , microbiology and biotechnology , microtubule , gene
Formation of tubulin/Na + ,K + ‐ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development.