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Low‐intensity pulsed ultrasound‐induced ATP increases bone formation via the P2X7 receptor in osteoblast‐like MC3T3‐E1 cells
Author(s) -
Manaka Soichiro,
Tanabe Natsuko,
Kariya Taro,
Naito Masako,
Takayama Tadahiro,
Nagao Mayu,
Liu Di,
Ito Koichi,
Maeno Masao,
Suzuki Naoto,
Miyazaki Masashi
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.12.013
Subject(s) - low intensity pulsed ultrasound , osteoblast , extracellular , chemistry , alkaline phosphatase , receptor , microbiology and biotechnology , adenosine triphosphate , bone remodeling , bone healing , endocrinology , medicine , biophysics , biochemistry , enzyme , anatomy , biology , ultrasound , therapeutic ultrasound , in vitro , radiology
Low‐intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS‐induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS‐induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS‐induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.