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MicroRNA‐134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins
Author(s) -
Gao Yongtao,
Liu Te,
Huang Yongyi
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.12.002
Subject(s) - microrna , notch signaling pathway , cancer research , endometrial cancer , biology , cancer stem cell , microbiology and biotechnology , cancer cell , cancer , in vitro , stem cell , gene , signal transduction , genetics
We aimed to ascertain the role of microRNAs (miRNAs) in regulating human endometrial cancer stem cells (HuECSCs). The expression level of miRNA‐134 (miR‐134), a member of the DLK1‐DIO3 genomic imprinted miRNA cluster, differed significantly between HuECSCs and human endometrial cancer cells (HuECCs). miR‐134 inhibited HuECSCs proliferation and migration by targeting protein O ‐glucosyltransferase 1 (POGLUT1) expression. Exogenous miR‐134 overexpression downregulated POGLUT1 and Notch pathway proteins in HuECSCs in vitro. miR‐134 overexpression affected the G2/M phase of HuECSCs and suppressed the growth of xenograft tumours formed. Thus, endogenous miR‐134 regulation in HuECSCs may suppress tumourigenesis in human endometrial carcinoma.