Identification of the glucosyltransferase that mediates direct flavone  C ‐glucosylation in  Gentiana triflora | Zendy

Sasaki Nobuhiro | Zendy; Nishizaki Yuzo | Zendy; Yamada Eri | Zendy; Tatsuzawa Fumi | Zendy; Nakatsuka Takashi | Zendy; Takahashi Hideyuki | Zendy; Nishihara Masahiro | Zendy

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Identification of the glucosyltransferase that mediates direct flavone C ‐glucosylation in Gentiana triflora

Author(s) -

Sasaki Nobuhiro,

Nishizaki Yuzo,

Yamada Eri,

Tatsuzawa Fumi,

Nakatsuka Takashi,

Takahashi Hideyuki,

Nishihara Masahiro

Publication year - 2015

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2014.11.045

Subject(s) - glucosyltransferase , isoorientin , chemistry , gentiana , petal , luteolin , biochemistry , flavonoid , glucoside , biology , botany , enzyme , medicine , alternative medicine , pathology , antioxidant

The major flavonoids accumulated in leaves of Japanese gentian ( Gentiana triflora ) were determined as isoorientin (luteolin 6‐ C ‐glucoside) and isoorientin 4′‐glucoside. A cDNA ( GtUF6CGT1 ) was isolated that encoded the UDP‐glucose‐dependent glucosyltransferase that is involved in C ‐glycosylflavone biosynthesis. The recombinant GtUF6CGT1 protein could transfer a glucose group to the C6 position of a flavone skeleton through C‐linkage, using UDP‐glucose as the glucosyl donor. These C ‐glycosylflavones also accumulated in petals. A good correlation was observed between GtUF6CGT1 expression and C ‐glycosylflavone accumulation in leaves and petals. GtUF6CGT1 is the first reported C ‐glucosyltransferase that mediates direct C ‐glucosylation of the flavone skeleton.

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