ROG1  encodes a monoacylglycerol lipase in  Saccharomyces cerevisiae | Zendy

Vishnu Varthini Lakshmanaperumal | Zendy; Selvaraju Kandasamy | Zendy; Srinivasan Malathi | Zendy; Nachiappan Vasanthi | Zendy

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ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae

Author(s) -

Vishnu Varthini Lakshmanaperumal,

Selvaraju Kandasamy,

Srinivasan Malathi,

Nachiappan Vasanthi

Publication year - 2015

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2014.11.019

Subject(s) - monoacylglycerol lipase , lipase , saccharomyces cerevisiae , biochemistry , diacylglycerol lipase , lipoprotein lipase , triglyceride lipase , chemistry , enzyme , cytosol , biology , gene , endocannabinoid system , receptor

Lipid metabolism is extensively studied in Saccharomyces cerevisiae . Here, we report that revertant of glycogen synthase kinase mutation‐1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae . The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 ( yju3 Δ rog1 Δ) and by site‐directed mutagenesis of the ROG1 lipase motif (G X S X G). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1‐ deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.

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