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ROG1 encodes a monoacylglycerol lipase in Saccharomyces cerevisiae
Author(s) -
Vishnu Varthini Lakshmanaperumal,
Selvaraju Kandasamy,
Srinivasan Malathi,
Nachiappan Vasanthi
Publication year - 2015
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.11.019
Subject(s) - monoacylglycerol lipase , lipase , saccharomyces cerevisiae , biochemistry , diacylglycerol lipase , lipoprotein lipase , triglyceride lipase , chemistry , enzyme , cytosol , biology , gene , endocannabinoid system , receptor
Lipid metabolism is extensively studied in Saccharomyces cerevisiae . Here, we report that revertant of glycogen synthase kinase mutation‐1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae . The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 ( yju3 Δ rog1 Δ) and by site‐directed mutagenesis of the ROG1 lipase motif (G X S X G). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1‐ deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.