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Epigenetic regulation of the NR4A orphan nuclear receptor NOR1 by histone acetylation
Author(s) -
Zhao Yue,
Nomiyama Takashi,
Findeisen Hannes M.,
Qing Hua,
Aono Jun,
Jones Karrie L.,
Heywood Elizabeth B.,
Bruemmer Dennis
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.11.017
Subject(s) - hdac4 , sap30 , creb , histone deacetylase 2 , hdac11 , histone deacetylase , acetylation , histone deacetylase 5 , hdac8 , histone h2a , creb binding protein , histone h3 , histone , chemistry , microbiology and biotechnology , biology , cancer research , transcription factor , biochemistry , gene
The nuclear receptor NOR1 is an immediate‐early response gene implicated in the transcriptional control of proliferation. Since the expression level of NOR1 is rapidly induced through cAMP response element binding (CREB) protein‐dependent promoter activation, we investigated the contribution of histone acetylation to this transient induction. We demonstrate that NOR1 transcription is induced by histone deacetylase (HDAC) inhibition and by depletion of HDAC1 and HDAC3. HDAC inhibition activated the NOR1 promoter, increased histone acetylation and augmented the recruitment of phosphorylated CREB to the promoter. Furthermore, HDAC inhibition increased Ser133 phosphorylation of CREB and augmented NOR1 protein stability. These data outline previously unrecognized mechanisms of NOR1 regulation and illustrate a key role for histone acetylation in the rapid induction of NOR1 .