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CXCL14 is no direct modulator of CXCR4
Author(s) -
Otte Maik,
Kliewer Andrea,
Schütz Dagmar,
Reimann Christiane,
Schulz Stefan,
Stumm Ralf
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.11.009
Subject(s) - cxcl14 , cxc chemokine receptors , microbiology and biotechnology , chemokine receptor , internalization , chemokine , jurkat cells , chemotaxis , phosphorylation , cxcr4 , signal transduction , chemistry , biology , cancer research , receptor , biochemistry , immunology , immune system , t cell
C‐X‐C motif chemokine 12/C‐X‐C chemokine receptor type 4 (CXCL12/CXCR4) signaling is involved in ontogenesis, hematopoiesis, immune function and cancer. Recently, the orphan chemokine CXCL14 was reported to inhibit CXCL12‐induced chemotaxis – probably by allosteric modulation of CXCR4. We thus examined the effects of CXCL14 on CXCR4 regulation and function using CXCR4‐transfected human embryonic kidney (HEK293) cells and Jurkat T cells. CXCL14 did not affect dose–response profiles of CXCL12‐induced CXCR4 phosphorylation, G protein‐mediated calcium mobilization, dynamic mass redistribution, kinetics of extracellular signal‐regulated kinase 1 (ERK1) and ERK2 phosphorylation or CXCR4 internalization. Hence, essential CXCL12‐operated functions of CXCR4 are insensitive to CXCL14, suggesting that interactions of CXCL12 and CXCL14 pathways depend on a yet to be identified CXCL14 receptor.