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MiR‐92a inhibits porcine ovarian granulosa cell apoptosis by targeting Smad7 gene
Author(s) -
Liu Jiying,
Yao Wang,
Yao Yong,
Du Xing,
Zhou Jilong,
Ma Baiquan,
Liu Honglin,
Li Qifa,
Pan Zengxiang
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.10.021
Subject(s) - gene knockdown , apoptosis , luciferase , reporter gene , granulosa cell , transfection , microbiology and biotechnology , transforming growth factor , signal transduction , cancer research , biology , microrna , gene expression , gene , chemistry , ovary , endocrinology , biochemistry
Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine‐tunes transforming growth factor β (TGFβ) signaling. This study demonstrates that miR‐92a regulates GC apoptosis in pig ovaries by targeting Smad7 directly. The expression level of miR‐92a was down‐regulated in atretic porcine follicles, whereas miR‐92a expression led to inhibition of GC apoptosis. The Smad7 gene was identified as a direct target of miR‐92a using a dual‐luciferase reporter assay. Transfection of GCs with miR‐92a mimics decreased Smad7 mRNA and protein levels, whereas expression of an miR‐92a inhibitor in GCs had the opposite effect. In addition, knockdown of Smad7 prevented GC apoptosis in cells that expressed the miR‐92a inhibitor.