Premium
Membrane interactions and fibrillization of α‐synuclein play an essential role in membrane disruption
Author(s) -
Chaudhary Himanshu,
Stefanovic Anja N.D.,
Subramaniam Vinod,
Claessens Mireille M.A.E.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.10.016
Subject(s) - membrane , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology
We studied α‐synuclein (αS) aggregation in giant vesicles, and observed dramatic membrane disintegration, as well as lipid incorporation into micrometer‐sized suprafibrillar aggregates. In the presence of dye‐filled vesicles, dye leakage and fibrillization happen concurrently. However, growing fibrils do not impair the integrity of phospholipid vesicles that have a low affinity for αS. Seeding αS aggregation accelerates dye leakage, indicating that oligomeric species are not required to explain the observed effect. The evolving picture suggests that fibrils that appear in solution bind membranes and recruit membrane‐bound monomers, resulting in lipid extraction, membrane destabilization and the formation of lipid‐containing suprafibrillar aggregates.