Premium
Reactive oxygen species increase HEPN1 expression via activation of the XBP1 transcription factor
Author(s) -
Kim Hyeon Seop,
Jung Guhung
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.10.011
Subject(s) - downregulation and upregulation , reactive oxygen species , transcription factor , activator (genetics) , apoptosis , microbiology and biotechnology , messenger rna , chemistry , gene expression , biology , gene , biochemistry
Hepatocellular carcinoma downregulated 1 ( HEPN1 ), a cell growth arrest‐ and apoptosis‐related gene, is suppressed in hepatocellular carcinoma (HCC). However, transcriptional control of HEPN1 has not been characterized. Here, we show that exposure to reactive oxygen species (ROS) leads to upregulation of the mRNA expression of HEPN1 in HCC cell lines. Mechanistically, ROS increase production of an alternately spliced form of X‐box binding protein 1 (XBP1s) and XBP1s increases HEPN1 expression by binding to the HEPN1 promoter, thereby acting as a transcriptional activator. Finally, HEPN1 overexpression increases the expression of p53, p21, and Bax, all of which are ROS‐upregulated proteins.