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Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts
Author(s) -
Ichinose Mizuho,
Uchida Masato,
Sugita Mamoru
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.09.031
Subject(s) - pentatricopeptide repeat , physcomitrella patens , rna editing , rna , chloroplast , biology , microbiology and biotechnology , gene , genetics , mutant
The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW‐subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P . patens . Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14 ‐C2 site, whereas over‐expression of PpPPR_45 increased the levels of RNA editing at both the rps14 ‐C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites.