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Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9
Author(s) -
Cheng Ranran,
Peng Jin,
Yan Yonghong,
Cao Peili,
Wang Jiewei,
Qiu Chen,
Tang Lichun,
Liu Di,
Tang Li,
Jin Jianping,
Huang Xingxu,
He Fuchu,
Zhang Pumin
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.09.008
Subject(s) - crispr , genome editing , cas9 , biology , gene , genetic enhancement , gene delivery , genome , viral vector , computational biology , gene knockout , adenoviridae , virology , genetics , recombinant dna
We developed an adenovirus‐based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue‐specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus‐based CRISPR/Cas9 system will find applications in a variety of experimental settings.