Premium
TR4 promotes fatty acid synthesis in 3T3‐L1 adipocytes by activation of pyruvate carboxylase expression
Author(s) -
Park Sung-Soo,
Kim Seung-Jin,
Choi Hojung,
Chang Chawnshang,
Kim Eungseok
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.09.007
Subject(s) - pyruvate carboxylase , fatty acid synthesis , 3t3 l1 , gene knockdown , acetyl coa carboxylase , fatty acid , fatty acid synthase , chemistry , beta oxidation , biochemistry , gene expression , phenylacetic acid , microbiology and biotechnology , biology , gene , enzyme , adipogenesis
We show that testicular orphan nuclear receptor 4 (TR4) increases the expression of pyruvate carboxylase (PC) gene in 3T3‐L1 adipocytes by direct binding to a TR4 responsive element in the murine PC promoter. While TR4 overexpression increased PC activity, oxaloacetate (OAA) and glycerol levels with enhanced incorporation of 14 C from 14 C‐pyruvate into fatty acids in 3T3‐L1 adipocytes, PC knockdown by short interfering RNA (siRNA) or inhibition of PC activity by phenylacetic acid (PAA) abolished TR4‐enhanced fatty acid synthesis. Moreover, TR4 microRNA reduced PC expression with decreased fatty acid synthesis in 3T3‐L1 adipocytes, suggesting that TR4‐mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene.